SPECIFICITY AND T-CELL RECEPTOR-BETA CHAIN USAGE OF A HUMAN COLLAGEN TYPE-II-REACTIVE T-CELL CLONE DERIVED FROM A HEALTHY INDIVIDUAL

Collagen type II (CII) is a cartilage-specific matrix compound well known as an inducer of an experimental, T cell-dependent autoimmune arthritis, a disease which shows some similarities to human rheumatoid arthritis. Here we report on an HLA-DR7-restricted human CD4 T cell clone (TC9), which was isolated from a healthy donor and recognizes human CII. After screening CNBr fragments of CII and tryptic fragments derived thereof, the T cell epitope could be mapped to amino acid residues 271-285 of the triple helical region of CII that are located within CNBr fragment 11 [alpha-1 (II) CB11]. This epitope was confirmed by a synthetic peptide stimulatory for TC9. The T cell receptor beta-chain of TC9 was cloned using the polymerase chain reaction; it comprises V(beta)6.7 and contains besides J(beta)2.3 and C(beta)2 an as yet undescribed sequence for the D segment.