FRACTIONATION AND VISCOMETRIC CHARACTERIZATION OF A (1-]3),(1-]4)-BETA-D-GLUCAN FROM OAT, AND UNIVERSAL CALIBRATION OF A HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHIC SYSTEM BY THE USE OF FRACTIONATED BETA-GLUCANS, ALGINATES AND PULLULANS

被引：34

作者：

VARUM, KM

MARTINSEN, A

SMIDSROD, O

机构：

[1] Norwegian Biopolymer Laboratory (NOBIPOL), Division of Biotechnology, Norwegian Institute of Technology, University of Trondheim, Trondheim

来源：

FOOD HYDROCOLLOIDS | 1991年 / 5卷 / 04期

关键词：

D O I：

10.1016/S0268-005X(09)80048-9

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A polydisperse sample of mixed-linkage (1 --> 3),(1 --> 4)-beta-D-glucans from oat aleurone was fractionated by conventional size exclusion chromatography on semipreparative columns of Sepharose CL 4B and 6B to produce fractions of low polydispersity. The fractions were characterized by their intrinsic viscosities and their number-average molecular weight. The HPSEC-chromatograms of the beta-glucan fractions suggested very narrow molecular weight distributions in all fractionated samples. From knowledge of the relative weights of the fractions and by assuming that each fraction was strictly monodisperse, the weight and number-average molecular weight of the unfractionated sample of beta-glucan were determined to be 71 900 and 49 000, respectively, in reasonable agreement with the number-average molecular weight determined by osmometry (55 000). The Mark-Houwink-Sakurada viscosity equation for. monodisperse beta-glucans was determined for samples having molecular weights from 26 x 10(3) to 137 x 10(3) g/mol as [eta] = 6.7 x 10(-2) M(n)0.75BAR (in ml/g) (20-degrees-C, 0.1 mol/dm3 NaCl). The beta-glucan fractions were further characterized by high-performance size-exclusion chromatography, and compared with commercially prepared pullulan fractions of low polydispersity and alginate fractions obtained in another study in this laboratory. The validity of the universal calibration procedure was tested with the three different polysaccharides, showing the same linear relationship between hydrodynamic volume ([eta] x M) and elution volume for all three polysaccharides. The commercial pullulan samples seemed well-suited for calibration purposes.

引用

页码：363 / 374

页数：12

共 25 条

[1]

Wood P.J., Anderson J.W., Braaten J.T., Cave N.A., Scott F.W., Vachon C., Cereal Foods World, 34, pp. 878-882, (1989)

[2]

Gould M.R., Anderson J.W., O'Mahony S., Cereals for Food and Beverages, (1980)

[3]

Harding S., Varum K.M., Stokke B.T., Smidsrod O., Advances in Carbohydrate Chemistry, 1, (1991)

[4]

Engster M., Abraham R., Toxicol. Appl. Pharmacol., 38, (1976)

[5]

Grubisic Z., Rempp P., Benoit P., J. Polym. Sci., Polym. Lett. Ed., 5, (1967)

[6]

Wu A.C.M., Bough W.A., Conrad E.C., Alden K.E., J. Chromatogr., 128, (1976)

[7]

Goto A., Araki C., Izumi I., Proc. Int. Soc. Citric., 2, (1981)

[8]

Marti M., De La Torre M.C., Am. J. Enol. Vitic., 40, pp. 221-223, (1989)

[9]

Terbojevich M., Cosani A., Palumbo M., Pregnolato F., Carbohydr. Res., 157, pp. 269-270, (1986)

[10]

Martinsen A., Skjak-Braek G., Smidsrod O., Zanetti F., Paoletti S., Comparison of different methods for determination of molecular weight and molecular weight distribution of alginates, Carbohydrate Polymers, 15, (1991)

← 1 2 3 →