A NOVEL CERAMIDE TRIHEXOSIDE FROM THE EGGS OF THE SEA-URCHIN, HEMICENTROTUS-PULCHERRIMUS

被引：11

作者：

KUBO, H

JIANG, GJ

IRIE, A

MORITA, M

MATSUBARA, T

HOSHI, M

机构：

[1] TOKYO INST TECHNOL,DEPT LIFE SCI,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

[2] TOKYO INST TECHNOL,GENE RES CTR,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

[3] KINKI UNIV,FAC SCI & TECHNOL,DEPT CHEM,HIGASHIOSAKA,OSAKA 557,JAPAN

[4] KINKI UNIV,SCI & TECHNOL RES INST,HIGASHIOSAKA,OSAKA 557,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1992年 / 111卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123826

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucosylceramide (Glc-beta-1-1Cer) and a novel ceramide trihexoside (Gal-beta-1-6Gal-beta-1-6Glc-beta-1-1Cer) were purified from the eggs of the sea urchin, Hemicentrotus pulcherrimus. Their chemical structures were determined by gas-liquid chromatography, methylation analysis, chromic acid oxidation, enzymatic hydrolysis, enzyme-linked immunosorbent assay, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectroscopy. The ceramide trihexoside has a novel carbohydrate structure, and its core structure, Gal-beta-1-6Glc, is also novel. The ceramide moieties of these glycolipids are almost identical. Two fatty acids, 22:1 and 22h:1, constitute more than 80% of the total acids. Long-chain bases are all phytosphingosine, approximately 90% of which is n-t18:0. The finding of melibiosylceramide (Gal-alpha-1-6Glc-beta-1-1Cer) from the eggs of another sea urchin species [Kubo,H. et al.(1988) J. Biochem. 104,755-760] and the present finding of the novel ceramide trihexoside suggest that there are a variety of unique sugar structures in sea urchin glycosphingolipids.

引用

页码：726 / 731

页数：6

共 31 条

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