SULFOLIPIDS AND GLYCOLIPID SULFOTRANSFERASE ACTIVITIES IN HUMAN RENAL-CELL CARCINOMA-CELLS

被引：55

作者：

KOBAYASHI, T

HONKE, K

KAMIO, K

SAKAKIBARA, N

GASA, S

MIYAO, N

TSUKAMOTO, T

ISHIZUKA, I

MIYAZAKI, T

MAKITA, A

机构：

[1] HOKKAIDO UNIV,SCH MED,INST CANC,BIOCHEM LAB,KITA KU,N15 W7,SAPPORO,HOKKAIDO 060,JAPAN

[2] SAPPORO MED COLL,DEPT UROL,SAPPORO,HOKKAIDO 060,JAPAN

[3] TEIKYO UNIV,DEPT BIOCHEM,TOKYO 173,JAPAN

[4] HOKKAIDO UNIV,SCH MED,DEPT INTERNAL MED 3,SAPPORO,HOKKAIDO 060,JAPAN

来源：

BRITISH JOURNAL OF CANCER | 1993年 / 67卷 / 01期

关键词：

D O I：

10.1038/bjc.1993.12

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A cell line (SMKT-R3) established from human renal cell carcinoma was characterised for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with S-35-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves.

引用

页码：76 / 80

页数：5

共 32 条

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