ACTIN DEPOLYMERIZING FACTOR IS A COMPONENT OF SLOW AXONAL-TRANSPORT

被引：22

作者：

BRAY, JJ ^{[1
]}

FERNYHOUGH, P ^{[1
]}

BAMBURG, JR ^{[1
]}

BRAY, D ^{[1
]}

机构：

[1] MRC,CELL BIOPHYS UNIT,LONDON WC1E 6AS,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1992年 / 58卷 / 06期

关键词：

ACTIN DEPOLYMERIZING FACTOR; ACTIN; AXONS; SLOW AXONAL TRANSPORT;

D O I：

10.1111/j.1471-4159.1992.tb10949.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the low molecular weight proteins transported with actin in the chicken sciatic nerve after injection of [S-35]methionine into the lumbar spinal cord. A prominent component of slow axonal transport with apparent molecular mass 19 kDa comigrated on two-dimensional gels with chicken actin depolymerizing factor (ADF), previously shown to be a major actin-binding protein in brain. There was comparatively little radioactivity associated with the actin monomer sequestering proteins, profilin or cofilin, and examination of the rapid component of axonal transport failed to reveal appreciable quantities of actin, ADF, profilin, or cofilin. These results show that both actin and ADF are carried by slow axonal transport and raise the possibility that actin travels within the axon in an unpolymerized form in a complex with ADF.

引用

页码：2081 / 2087

页数：7

共 48 条

[1] SEQUENCE OF CDNAS ENCODING ACTIN DEPOLYMERIZING FACTOR AND COFILIN OF EMBRYONIC CHICKEN SKELETAL-MUSCLE - 2 FUNCTIONALLY DISTINCT ACTIN-REGULATORY PROTEINS EXHIBIT HIGH STRUCTURAL HOMOLOGY [J].