INDUCTION OF THE GENES RAD54 AND RNR2 BY VARIOUS DNA-DAMAGING AGENTS IN SACCHAROMYCES-CEREVISIAE

被引:35
作者
AVERBECK, D
AVERBECK, S
机构
[1] Institute Curie-Section de Biologie, URA 1292 CNRS, F-75231 Paris Cedex 05, 26, Fue d'Ulm
来源
MUTATION RESEARCH-DNA REPAIR | 1994年 / 315卷 / 02期
关键词
FUROCOUMARINS; GENE INDUCTION; SACCHAROMYCES CEREVISIAE; GAMMA-RAYS; 254 NM UV; OXIDATIVE DNA DAMAGE;
D O I
10.1016/0921-8777(94)90013-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The relationship between the induction of the genes RAD54 and RNR2 and the induction and repair of specific DNA lesions was studied in the yeast Saccharomyces cerevisiae using Rad54-lacZ and RNR2-lacZ fusion strains. Gene induction was followed by measuring beta-galactosidase activity. At comparable levels of furocoumarin-DNA photoadducts, RAD54 was more effectively induced by bifunctional than by monofunctional furocoumarins indicating that mixtures of monoadducts (MA) and interstrand cross-links (CL) provide a stronger inducing signal than MA. RNR2 induction kinetics were measured in relation to cell growth and survival responses after treatment with the furocoumarins 8-methoxypsoralen (8-MOP), 5-methoxypsoralen (5-MOP), 3-carbethoxypsoralen (3-CPs), 7-methylpyrido[3,4-c]psoralen (MePyPs) and 4,4',6-trimethylangelicin (TMA), benzo[a]pyrene (B(a)P and 1,6-dioxapyrene (1,6-DP) plus UVA, 254 nm UV radiation and cobalt-60 gamma-radiation. Induction of RNR2 took place during the DNA repair period before resumption of cell growth and clearly increased with increasing equitoxic dose levels. Treatments with furocoumarin plus 365 nm radiation (UVA) and 254 nm (UV) radiation were effective inducers whereas gene induction was relatively weak after gamma-radiation and absent after the induction of oxidative damage by B(a)P and 1,6-DP and UVA. The results suggest that it is the specific processing of different DNA lesions that determines the potency of the induction signal. Apparently, DNA lesions such as CL, and probably also closely located MA or pyrimidine dimers in opposite DNA strands involving the formation of double-strand breaks as repair intermediates, are most effective inducers.
引用
收藏
页码:123 / 138
页数:16
相关论文
共 67 条
  • [1] SEMIDOMINANT SUPPRESSORS OF SRS2 HELICASE MUTATIONS OF SACCHAROMYCES-CEREVISIAE MAP IN THE RAD51 GENE, WHOSE SEQUENCE PREDICTS A PROTEIN WITH SIMILARITIES TO PROKARYOTIC RECA PROTEINS
    ABOUSSEKHRA, A
    CHANET, R
    ADJIRI, A
    FABRE, F
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (07) : 3224 - 3234
  • [2] A YEAST PROTEIN ANALOGOUS TO ESCHERICHIA-COLI RECA PROTEIN WHOSE CELLULAR-LEVEL IS ENHANCED AFTER UV IRRADIATION
    ANGULO, JF
    SCHWENCKE, J
    MOREAU, PL
    MOUSTACCHI, E
    DEVORET, R
    [J]. MOLECULAR & GENERAL GENETICS, 1985, 201 (01): : 20 - 24
  • [3] NEW ASPECTS OF THE REPAIR AND GENOTOXICITY OF PSORALEN PHOTOINDUCED LESIONS IN DNA
    AVERBECK, D
    DARDALHON, M
    MAGANASCHWENCKE, N
    MEIRA, LB
    MENIEL, V
    BOITEUX, S
    SAGE, E
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 14 (1-2) : 47 - 63
  • [4] REPAIR OF FUROCOUMARIN-PLUS-UVA-INDUCED DAMAGE AND MUTAGENIC CONSEQUENCES IN EUKARYOTIC CELLS
    AVERBECK, D
    DARDALHON, M
    MAGANASCHWENCKE, N
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1990, 6 (1-2) : 221 - 236
  • [5] PHOTOBIOLOGICAL ACTIVITIES OF 1,6-DIOXAPYRENE IN PROKARYOTIC AND EUKARYOTIC CELLS
    AVERBECK, D
    POLASA, K
    BUISSON, JP
    BENSASSON, R
    ROUGEE, M
    CADET, J
    RAVANAT, JL
    PERIN, F
    VIGNY, P
    DEMERSEMAN, P
    [J]. MUTATION RESEARCH, 1993, 287 (02): : 165 - 179
  • [6] RELATIONSHIP BETWEEN LESIONS PHOTOINDUCED BY MONO-FUNCTIONAL AND BI-FUNCTIONAL FUROCOUMARINS IN DNA AND GENOTOXIC EFFECTS IN DIPLOID YEAST
    AVERBECK, D
    [J]. MUTATION RESEARCH, 1985, 151 (02): : 217 - 233
  • [7] AVERBECK D, 1990, Yeast, V6, pS114
  • [8] BACCICHETTI F, 1984, PHOTOCHEM PHOTOBIOL, V39, P525
  • [9] INDUCIBLE NUCLEOTIDE EXCISION REPAIR IN NEUROSPORA
    BAKER, TI
    [J]. MOLECULAR & GENERAL GENETICS, 1983, 190 (02): : 295 - 299
  • [10] THE NUCLEOTIDE-SEQUENCE OF THE DNA-LIGASE GENE (CDC9) FROM SACCHAROMYCES-CEREVISIAE - A GENE WHICH IS CELL-CYCLE REGULATED AND INDUCED IN RESPONSE TO DNA DAMAGE
    BARKER, DG
    WHITE, JHM
    JOHNSTON, LH
    [J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (23) : 8323 - 8337