LOSS OF MICROTUBULES IN THE INTERPHASE CELLS OF ONION (ALLIUM-CEPA L) ROOT-TIPS FROM THE CELL CORTEX AND THEIR APPEARANCE IN THE CYTOPLASM AFTER TREATMENT WITH CYCLOHEXIMIDE

As part of a project to investigate the mechanism of cortical microtubule (MT) alignment, we examined the effects of cycloheximide (CHM) on cortical MTs in the root tip cells of allium cepa L. Results show that although a preprophase band of MTs remained in the cell cortex, interphase MTs disappeared from the cortical cytoplasm and then appeared concomitantly in the inner cytoplasm when the rate of de novo protein synthesis was reduced with CHM (11-360 mu m for 2 h).