SYNTHESIS OF HIGH-MOLECULAR-WEIGHT POLY([R]-(-)-3-HYDROXYBUTYRATE) IN TRANSGENIC ARABIDOPSIS-THALIANA PLANT-CELLS

被引：41

作者：

POIRIER, Y

SOMERVILLE, C

SCHECHTMAN, LA

SATKOWSKI, MM

NODA, I

机构：

[1] CARNEGIE INST WASHINGTON,DEPT PLANT BIOL,STANFORD,CA 94305

[2] PROCTER & GAMBLE CO,MIAMI VALLEY LABS,CINCINNATI,OH 45253

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1995年 / 17卷 / 01期

基金：

美国国家科学基金会;

关键词：

POLYHYDROXYBUTYRATE; POLYHYDROXYALKANOATE; ARABIDOPSIS THALIANA;

D O I：

10.1016/0141-8130(95)93511-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-molecular-weight poly([R]-(-)-3-hydroxybutyrate) (PHB), a biodegradable thermoplastic, was produced from a suspension culture of transgenic Arabidopsis thaliana plant cells expressing two genes from the bacterium Alcaligenes eutrophus involved in the synthesis of PHB. The molecular structure of the plant-produced polymer was analysed by gas chromatography, mass spectrometry, proton nuclear magnetic resonance spectroscopy, infra-red spectroscopy, spectropolarimetry, differential scanning calorimetry, X-ray diffraction and size exclusion chromatography. The results indicate that the polymer from transgenic plants appears to have a chemical structure identical to that of PHB produced by bacteria. However, the molecular weight distribution of the plant-produced PHB was much broader than that of typical bacterial PHB.

引用

页码：7 / 12

页数：6

共 16 条

[1] SYNTHESIS AND CHARACTERIZATION OF POLY-BETA-HYDROXYBUTYRATE .1. SYNTHESIS OF CRYSTALLINE DL-POLY-BETA-HYDROXYBUTYRATE FROM DL-BETA-BUTYROLACTONE [J].