INVERSE DEUTERIUM KINETIC ISOTOPE EFFECT FOR PEROXIDATION IN HUMAN LOW-DENSITY-LIPOPROTEIN (LDL) - A SIMPLE TEST FOR TOCOPHEROL-MEDIATED PEROXIDATION OF LDL LIPIDS

被引：60

作者：

WITTING, PK ^{[1
]}

BOWRY, VW ^{[1
]}

STOCKER, R ^{[1
]}

机构：

[1] HEART RES INST, BIOCHEM GRP, SYDNEY, NSW 2050, AUSTRALIA

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

ANTIOXIDANT; ATHEROSCLEROSIS; OXIDATION; VITAMIN-E;

D O I：

10.1016/0014-5793(95)01172-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Tocopherol (alpha-TOH) can act as a pro- or antioxidant for isolated ubiquinol-10-free human low density lipoprotein (LDL). We demonstrate that alpha-TOH is a more potent prooxidant than other forms of vitamin E for LDL peroxidation initiated by mild fluxes of aqueous peroxyl radicals and low concentrations of Cu2+. A simple deuterium exchange test shows that alpha-TOH switches from pro- to anti-oxidant at Cu2+:LDL ratios > 2.5. The results suggest that this test may be useful to distinguish 'inhibited' peroxidation of emulsion lipids propagated via the lipid peroxyl radical from that mediated via the antioxidant radical.

引用

页码：45 / 49

页数：5

共 28 条

[1]

Burton, Ingold, Acc. Chem. Res., 19, pp. 194-201, (1986)

[2]

Burton, Joyce, Ingold, Arch. Biochem. Biophys., 221, pp. 281-290, (1983)

[3]

Esterbauer, Jurgens, Quehenberger, Koller, J. Lipid Res., 28, pp. 495-509, (1987)

[4]

Esterbauer, Gebicki, Puhl, Jurgens, Free Radic. Biol. Med., 13, pp. 341-390, (1992)

[5]

Stocker, Curr. Opin. Lipidol., 5, pp. 422-433, (1994)

[6]

Jessup, Rankin, De Whalley, Hoult, Scott, Leake, Biochem. J., 265, pp. 399-405, (1990)

[7]

Dieber-Rotheneder, Puhl, Waeg, Striegl, Esterbauer, J. Lipid Res., 32, pp. 1325-1332, (1991)

[8]

Jialal, Grundy, J. Lipid Res., 33, pp. 899-906, (1992)

[9]

Harats, Ben-Naim, Dabach, Hollander, Havivi, Stein, Stein, Atherosclerosis, 85, pp. 47-54, (1990)

[10]

Princen, van Poppel, Vogelezang, Buytenhek, Kok, Arterioscler. Thromb., 12, pp. 554-562, (1992)

← 1 2 3 →