2 THYMIDYLATE SYNTHETASES IN BACILLUS-SUBTILIS

被引：51

作者：

NEUHARD, J

PRICE, AR

SCHACK, L

THOMASSEN, E

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1978年 / 75卷 / 03期

关键词：

D O I：

10.1073/pnas.75.3.1194

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacillus subtilis grown at temperatures below 37° contains two thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46° they only contain TSaseA activity. This allows an easy positive selection for thyA and thyA,thyB mutants. The two TSases have been physically separated, and they show similar overall requirements for activity. However, they differ significantly in both their kinetic and their physicochemical properties.

引用

页码：1194 / 1198

页数：5

共 19 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].