DETERMINATION OF 2ND MESSENGERS AND PROTEIN KINASE-C IN BONE-MARROW DERIVED MACROPHAGES STIMULATED WITH A BACTERIAL LIPOPEPTIDE

被引：16

作者：

HAUSCHILDT, S ^{[1
]}

WOLF, B ^{[1
]}

LUCKHOFF, A ^{[1
]}

BESSLER, WG ^{[1
]}

机构：

[1] INST ANGEW PHYSIOL,W-7800 FREIBURG,GERMANY

来源：

MOLECULAR IMMUNOLOGY | 1990年 / 27卷 / 06期

关键词：

D O I：

10.1016/0161-5890(90)90065-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthetic lipopeptide Pam3Cys-Ala-Gly, an analogue of the N-terminal part of bacterial lipoprotein, constitutes a potent macrophage activator. The role of protein kinase C (PKC) in lipopeptide induced signal transduction was investigated. As determined by enzymatic and immunochemical methods, translocation of PKC could not be observed in lipopeptide stimulated bone marow derived macrophages. Our studies showed that the membrane-associated form of PKC displayed different characteristics than the cytosolic form. The second messengers, inositoltrisphosphate, cAMP and cGMP, did not seem to be involved in signal transduction. Unlike LPS, Pam3Cys-Ala-Gly induced a rapid rise in cytosolic Ca2+ which was due to an influx of extracellular calcium as well as to a redistribution of intracellular calcium. The data suggest that one major intracellular signal transduction mechanism initiated by lipopeptide consists of altering internal Ca2+ concns. © 1990.

引用

页码：473 / 479

页数：7

共 29 条

[1] MOLECULAR TRANSDUCTIONAL MECHANISMS BY WHICH IFN-GAMMA AND OTHER SIGNALS REGULATE MACROPHAGE DEVELOPMENT [J].