SIMULTANEOUS DETERMINATION OF GINSENOSIDES AND SAIKOSAPONINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

KANAZAWA, H

NAGATA, Y

MATSUSHIMA, Y

TOMODA, M

TAKAI, N

机构：

[1] KYORITSU COLL PHARMACEUT SCI,SHIBAKOEN 1-5-30,MINATO KU,TOKYO 105,JAPAN

[2] UNIV TOKYO,INST IND SCI,MINATO KU,TOKYO 106,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 507卷

关键词：

D O I：

10.1016/S0021-9673(01)84210-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method. © 1990.

引用

页码：327 / 332

页数：6

共 6 条

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