SEPARATION OF RAT-TISSUE HISTONE H1 SUBTYPES BY REVERSE-PHASE HPLC - IDENTIFICATION AND ASSIGNMENT TO A STANDARD H1 NOMENCLATURE

被引：33

作者：

LINDNER, H

HELLIGER, W

PUSCHENDORF, B

机构：

[1] Inst. fur Medizinische Chemie, Universitat Innsbruck, A-6020 Innsbruck

来源：

BIOCHEMICAL JOURNAL | 1990年 / 269卷 / 02期

关键词：

D O I：

10.1042/bj2690359

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H1 histones from rat liver and rat testis were separated by reverse-phase h.p.l.c. Within 40 min six subfractions (H10, H1b, H1a, H1d, H1e + H1c and H1c) and seven subfractions (H10, H1b, H1a, H1d, H1e + H1c, H1c and H1t) respectively were isolated by using a linear acetonitrile gradient. Each individual H1 subtype was identified either by comparing the H1 variants (contained in both tissues but in different quantities) or by SDS/PAGE and acetic acid/urea/PAGE. Moreover, all H1 variants were characterized by amino acid analyses. The amino acid compositions of rat histone subfractions H10, H1b and H1e were determined for the first time. It was possible to classify unambiguously the H1 subfractions obtained by h.p.l.c. by following the standardized H1 nomenclature for electrophoretic systems recommended by Lennox, Oshima and Cohen [(1982) J. Biol. Chem. 257, 5183-5189]. Incorrect assignments that have been made in various publications are discussed.

引用

页码：359 / 363

页数：5

共 33 条

[1] NUCLEOSOMES ARE ASSEMBLED INTO DISCRETE SIZE STRUCTURES BY HISTONE H-1 INVITRO [J].