CRYSTAL-STRUCTURE OF PLASTOCYANIN FROM A GREEN-ALGA, ENTEROMORPHA-PROLIFERA

被引:134
作者
COLLYER, CA
GUSS, JM
SUGIMURA, Y
YOSHIZAKI, F
FREEMAN, HC
机构
[1] UNIV SYDNEY,DEPT INORGAN CHEM,SYDNEY,NSW 2006,AUSTRALIA
[2] TOHO UNIV,FAC SCI,FUNABASHI,CHIBA 274,JAPAN
关键词
D O I
10.1016/0022-2836(90)90269-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The crystal structure of the Cu-containing protein plastocyanin (Mr 10,500) from the green alga Enteromorpha prolifera has been solved by molecular replacement. The structure was refined by constrained-restrained and restrained reciprocal space least-squares techniques. The refined model includes 111 solvent sites. There is evidence for alternate conformers at eight residues. The residual is 0.12 for a data set comprising 74% of all observations accessible at 1.85 Å resolution. The β-sandwich structure of the algal plastocyanin is effectively the same as that of poplar leaf (Populus nigra var. italica) plastocyanin determined at 1.6 Å resolution. The sequence homology between the two proteins is 56%. Differences between the contacts in the hydrophobic core create some significant (0.5 to 1.2 Å) movements of the polypeptide backbone, resulting in small differences between the orientations and separations of corresponding β-strands. These differences are most pronounced at the end of the molecule remote from the Cu site. The largest structural differences occur in the single non-β strand, which includes the sole turn of helix in the molecule: two of the residues in a prominent kink of the poplar plastocyanin backbone are missing from the algal plastocyanin sequence, and there is a significant change in the position of the helical segment in relation to the β-sandwich. Several other small but significant structural differences can be correlated with intermolecular contacts in the crystals. An intramolecular carboxyl-carboxylate hydrogen bond in the algal plastocyanin may be associated with an unusually high pKa. The dimensions of the Cu site in the two plastocyanins are, within the limits of precision, identical. © 1990.
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页码:617 / 632
页数:16
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