L-ARGININE IS ESSENTIAL FOR THE FORMATION INVITRO OF ETHYLENE BY AN EXTRACT OF PSEUDOMONAS-SYRINGAE

被引:28
作者
NAGAHAMA, K
OGAWA, T
FUJII, T
TAZAKI, M
GOTO, M
FUKUDA, H
机构
[1] KUMAMOTO INST TECHNOL,DEPT APPL MICROBIAL TECHNOL,IKEDA 4-22-1,KUMAMOTO 860,JAPAN
[2] SHIZUOKA UNIV,FAC AGR,OYA,SHIZUOKA 422,JAPAN
[3] KUMAMOTO INST TECHNOL,DEPT IND CHEM,KUMAMOTO 860,JAPAN
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷
关键词
D O I
10.1099/00221287-137-7-1641
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A system was developed for the formation of ethylene in vitro by an extract of Pseudomonas syringae pv. phaseolicola PK2. The ethylene-forming activity of a cell-free extract of this bacterium measured in a system reported previously was almost completely lost when the cell-free extract was dialysed against potassium phosphate buffer for 24 at 4-degrees-C. When the fraction of cell-free extract with a molecular mass < 10 kDa (SupI) was added back to the enzyme fraction after gel filtration of the cell-free extract, the enzymic activity increased to about four times that of the gel-filtered crude enzyme. The action of SupI could be reproduced by the addition of L-arginine. The complete system for the formation of ethylene under aerobic conditions in vitro required 0.25 mM-2-oxoglutarate, 0.2 mM-FeSO4, 2 mM-DTT, 10 mM-L-histidine and 0.2 mM-L-arginine. The cofactor specificity was examined by replacing L-arginine or L-histidine with various analogues, but none of them were effective. The components of this system, with the exception of L-histidine, were similar to those of a system derived from the ethylene-producing, plant pathogenic fungus Penicillium digitatum which also produced ethylene in vitro in a reaction dependent on 2-oxoglutarate. The intermediates in the ethylene-forming reaction are postulated and the roles of L-arginine and L-histidine in the formation of ethylene by Ps. syringae are discussed.
引用
收藏
页码:1641 / 1646
页数:6
相关论文
共 13 条
[1]   MICROBIAL-PRODUCTION OF C-2-HYDROCARBONS, ETHANE, ETHYLENE AND ACETYLENE [J].
FUKUDA, H ;
FUJII, T ;
OGAWA, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (05) :1363-1365
[2]   ETHYLENE PRODUCTION FROM L-METHIONINE BY CRYPTOCOCCUS-ALBIDUS [J].
FUKUDA, H ;
TAKAHASHI, M ;
FUJII, T ;
OGAWA, T .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 67 (03) :173-175
[3]   AN NADH-FE(III)EDTA OXIDOREDUCTASE FROM CRYPTOCOCCUS-ALBIDUS - AN ENZYME INVOLVED IN ETHYLENE PRODUCTION INVIVO [J].
FUKUDA, H ;
TAKAHASHI, M ;
FUJII, T ;
TAZAKI, M ;
OGAWA, T .
FEMS MICROBIOLOGY LETTERS, 1989, 60 (01) :107-111
[4]  
FUKUDA H, 1989, FEMS MICROBIOL LETT, V59, P1, DOI 10.1016/S0378-1097(97)00516-8
[5]   PREPARATION OF A CELL-FREE ETHYLENE-FORMING SYSTEM FROM PENICILLIUM-DIGITATUM [J].
FUKUDA, H ;
FUJII, T ;
OGAWA, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (04) :977-981
[6]  
GOTO M, 1987, PLANT CELL PHYSIOL, V28, P405
[7]  
GOTO M, 1985, PLANT CELL PHYSIOL, V26, P141
[8]   ETHYLENE FORMATION BY CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI [J].
INCE, JE ;
KNOWLES, CJ .
ARCHIVES OF MICROBIOLOGY, 1986, 146 (02) :151-158
[9]  
MANSOURI S, 1989, J GEN MICROBIOL, V135, P2819
[10]   THE ROLE OF NADH-FE(III)EDTA OXIDOREDUCTASE IN ETHYLENE FORMATION FROM 2-KETO-4-METHYLTHIOBUTYRATE [J].
OGAWA, T ;
TAKAHASHI, M ;
FUJII, T ;
TAZAKI, M ;
FUKUDA, H .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1990, 69 (05) :287-291