COMPLEMENTATION OF THE AMYLOSE-FREE STARCH MUTANT OF POTATO (SOLANUM-TUBEROSUM) BY THE GENE ENCODING GRANULE-BOUND STARCH SYNTHASE

被引：29

作者：

VANDERLEIJ, FR ^{[1
]}

VISSER, RGF ^{[1
]}

OOSTERHAVEN, K ^{[1
]}

VANDERKOP, DAM ^{[1
]}

JACOBSEN, E ^{[1
]}

FEENSTRA, WJ ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT PLANT BREEDING,6700 AJ WAGENINGEN,NETHERLANDS

来源：

THEORETICAL AND APPLIED GENETICS | 1991年 / 82卷 / 03期

关键词：

SOLANUM-TUBEROSUM; AGROBACTERIUM-RHIZOGENES; STARCH; AMYLOSE-FREE; WAXY;

D O I：

10.1007/BF02190614

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Agrobacterium rhizogenes-mediated introduction of the wild-type allele of the gene encoding granule-bound starch synthase (GBSS) into the amylose-free starch mutant amf of potato leads to restoration of GBSS activity and amylose synthesis, which demonstrates that Amf is the structural gene for GBSS. Amylose was found in columella cells of root tips, in stomatal guard cells, tubers, and pollen, while in the control experiments using only vector DNA. these tissues remained amylose free. This confirms the fact that, in potato. GBSS is the only enzyme responsible for the presence of amylose. accumulating in all starch-containing tissues. Amylose-containing transformants showed no positive correlation between GBSS activity and amylose content, which confirms that the former is not the sole regulating factor in amylose metabolism.

引用

页码：289 / 295

页数：7

共 33 条

[1] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].

BEVAN, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[4]

CASSADABAN MJ, 1980, J MOL BIOL, V138, P174

[5]

Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]

[6] BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].

DITTA, G ;

STANFIELD, S ;

CORBIN, D ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351

[7]

FRANDSEN N O, 1968, Theoretical and Applied Genetics, V38, P153, DOI 10.1007/BF00933811

[8] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[9] ISOLATION OF AN AMYLOSE-FREE STARCH MUTANT OF THE POTATO (SOLANUM-TUBEROSUM-L) [J].

HOVENKAMPHERMELINK, JHM ;

JACOBSEN, E ;

PONSTEIN, AS ;

VISSER, RGF ;

VOSSCHEPERKEUTER, GH ;

BIJMOLT, EW ;

DEVRIES, JN ;

WITHOLT, B ;

FEENSTRA, WJ .

THEORETICAL AND APPLIED GENETICS, 1987, 75 (01) :217-221

[10] RAPID ESTIMATION OF THE AMYLOSE AMYLOPECTIN RATIO IN SMALL AMOUNTS OF TUBER AND LEAF TISSUE OF THE POTATO [J].

HOVENKAMPHERMELINK, JHM ;

DEVRIES, JN ;

ADAMSE, P ;

JACOBSEN, E ;

WITHOLT, B ;

FEENSTRA, WJ .

POTATO RESEARCH, 1988, 31 (02) :241-246

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