THE LIGHT-HARVESTING COMPLEX-II (B800/850) FROM RHODOSPIRILLUM-MOLISCHIANUM IS AN OCTAMER

被引：26

作者：

KLEINEKOFORT, W

GERMEROTH, L

VANDENBROEK, JA

SCHUBERT, D

MICHEL, H

机构：

[1] UNIV FRANKFURT,INST BIOPHYS,THEODOR STERN KAI 7,HAUS 74,W-6000 FRANKFURT 70,GERMANY

[2] MPI BIOPHYS,FRANKFURT,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1140卷 / 01期

关键词：

LIGHT-HARVESTING COMPLEX-II; STATE OF ASSOCIATION; ANALYTICAL ULTRACENTRIFUGATION; (R-MOLISCHIANUM);

D O I：

10.1016/0005-2728(92)90025-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molar mass of the light-harvesting complex II (LH II) from Rhodospirillum molischianum in solutions of two nonionic detergents (N,N-dimethyldodecylamine N-oxide, nonaethyleneglycol lauryl ether) was studied by sedimentation equilibrium experiments in the analytical ultracentrifuge. To account for the contribution of protein-bound detergent to the effective particle mass, solvent density was varied by addition of D2O. In both detergents, the relative molar mass of the protein/pigment complex was found to be 114500 +/- 7% (maximum error), corresponding to the (8.0 +/- 0.6)-fold of that of the monomeric unit. The complex is thus the octamer of the basic unit.

引用

页码：102 / 104

页数：3

共 15 条

[1] DETERMINATION OF THE AGGREGATE SIZE IN DETERGENT SOLUTION OF THE LIGHT-HARVESTING CHLOROPHYLL A/B-PROTEIN COMPLEX FROM CHLOROPLAST MEMBRANES [J].