EFFECTS OF AMINO-ACID SUBSTITUTIONS IN THE PROMOTER - 10 BINDING REGION OF THE SIGMA(A)-FACTOR ON GROWTH OF BACILLUS-SUBTILIS

被引：10

作者：

CHANG, BY ^{[1
]}

DOI, RH ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT BIOCHEM & BIOPHYS,DAVIS,CA 95616

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 08期

关键词：

D O I：

10.1128/JB.175.8.2470-2474.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

On the bases of structural and functional information about the Bacillus subtilis sigma(A) protein and the techniques of site-directed mutagenesis, we constructed a B. subtilis sigA mutant (DB1005) which grows normally at 37-degrees-C but is sensitive to higher temperatures. DNA sequencing analyses revealed that DB1005 has Ile-198-->Ala and Ile-202-->Ala amino acid substitutions in the alpha-helix of the 2.4 region of the sigma(A) protein. Western blotting (immunoblotting) revealed that this mutant sigma(A) protein is stable at both 37 and 49-degrees-C. These results suggest that Ile-198 and Ile-202 separately or in combination are critical for the sigma(A) protein to be functional at the restrictive temperature.

引用

页码：2470 / 2474

页数：5

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