EFFECT OF ANTIBIOTICS ON CODED BINDING OF PEPTIDYL-TRNA TO RIBOSOME AND ON TRANSFER OF PEPTIDYL RESIDUE TO PUROMYCIN

The effect of antibiotics on the coded binding of peptidyl‐tRNA to the ribosome and the transfer of the peptidyl residue from peptidyl‐tRNA to puromycin has been studied. The coded binding of polylysyl‐tRNA was inhibited only by tetracyclines, the binding of acetylphenyl‐alanyl‐tRNA was inhibited by tetracyclines and streptomycin and stimulated by spiramycin and carbomycin. The most potent inhibitors of the puromycin reaction with polylysyl‐tRNA were the macrolides erythromycin, spiramycin, carbomycin and oleandomycin, inhibiting by 50% at a concentration of 0.1 μM; neomycin (1 μM), chloramphenicol (100 μM), tetracyclines (100 μM), streptomycin (50 μM) and streptovitacin A (1 mM) were somewhat less effective. The puromycin reaction with acetylphenylalanyl‐tRNA was inhibited by antibiotics in a similar way, only erythromycin stimulated this reaction at concentrations between 0.1 and 500 μM. Copyright © 1969, Wiley Blackwell. All rights reserved