PHOSPHOFRUCTOKINASE ISOZYMES FROM HUMAN ORGANS AND BLOOD-CELLS

被引：3

作者：

ETIEMBLE, J ^{[1
]}

SIMEON, J ^{[1
]}

PICAT, C ^{[1
]}

BOIVIN, P ^{[1
]}

机构：

[1] HOP BEAUJON,CNRS,EQUIPE RECH 573,F-92118 CLICHY,FRANCE

来源：

ENZYME | 1979年 / 24卷 / 01期

关键词：

D O I：

10.1159/000458629

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphofructokinase (PFK) isozymes of blood cells and some human tissues were studied by starch gel electrophoresis and immunoprecipitation by anti-muscle and anti-erythrocyte PFK sera. PFK from muscle, heart, brain and placenta were totally precipitated by both antisera. PFK from blood cells (erythrocytes, lymphocytes, granulocytes, platelets) were precipitated more strongly by anti-erythrocyte PFK serum than by anti-muscle PFK serum. Liver, kidney and monoblast PFK were slightly precipitated by both antisera. From the electrophoretic patterns and the immunoprecipitation curves we may conclude that muscle contains the hormotetrameric M4 forms; platelet, liver and kidney the homotetrameric E4 form, and blood cells the M-E hybrids. Monoblasts probably contain a E4 type PFK precursor, and heart, placenta and brain, a modified M4 type PFK. Other isozymes, unrelated with muscle and erythrocyte, were revealed in liver and kidney.

引用

页码：61 / 66

页数：6

共 12 条

[1]

BOYUM A, 1974, TISSUE ANTIGENS, V4, P269

[2] HEREDITARY HEMOLYTIC-ANEMIA WITH ERYTHROCYTE PHOSPHOFRUCTOKINASE DEFICIENCY - STUDIES OF SOME PROPERTIES OF ERYTHROCYTE AND MUSCLE ENZYME [J].

ETIEMBLE, J ;

KAHN, A ;

BOIVIN, P ;

BERNARD, JF ;

GOUDEMAND, M .

HUMAN GENETICS, 1976, 31 (01) :83-91

[3] DISTRIBUTION OF PHOSPHOFRUCTOKINASE ISOENZYMES IN RABBIT, MOUSE, GUINEA-PIG AND RAT [J].

GONZALEZ, F ;

TSAI, MY ;

KEMP, RG .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1975, 52 (02) :315-319

[4]

KARADSHEH NS, 1977, J BIOL CHEM, V252, P3515

[5] MOLECULAR-BASIS OF MUSCLE PHOSPHOFRUCTOKINASE DEFICIENCY [J].

LAYZER, RB ;

RASMUSSEN, J .

ARCHIVES OF NEUROLOGY, 1974, 31 (06) :411-417

[6] MUSCLE PHOSPHOFRUCTOKINASE DEFICIENCY [J].

LAYZER, RB ;

ROWLAND, LP ;

RANNEY, HM .

ARCHIVES OF NEUROLOGY, 1967, 17 (05) :512-+

[7]

LAYZER RB, 1969, J BIOL CHEM, V244, P3823

[8] MULTIPLE ISOENZYMES OF HUMAN PHOSPHOFRUCTOKINASE [J].

LAYZER, RB ;

CONWAY, MM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 40 (06) :1259-&

[9] OKINASE IN RED-CELLS [J].

NIESSNER, H ;

BEUTLER, E .

BIOCHEMICAL MEDICINE, 1974, 9 (01) :73-76

[10] PHOSPHOFRUCTOKINASE DEFICIENCY IN SKELETAL MUSCLE - A NEW TYPE OF GLYCOGENOSIS [J].

TARUI, S ;

OKUNO, G ;

IKURA, Y ;

TANAKA, T ;

SUDA, M ;

NISHIKAW.M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 19 (04) :517-&

← 1 2 →