EFFECTS OF DAPI ON HUMAN-LEUKOCYTES INVITRO

被引：32

作者：

ROCCHI, A

DICASTRO, M

PRANTERA, G

机构：

来源：

CYTOGENETICS AND CELL GENETICS | 1979年 / 23卷 / 04期

关键词：

D O I：

10.1159/000131335

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DAPI (4'-6-diamidino-2-phenylindole), a fluorochrome specific for AT-rich DNA, was supplied for 24 h at various concentrations to human leukocytes in culture. This treatment caused the appearance on the chromosomes of specific areas lacking spiralization. In particular, the centrometric regions of chromosomes 1, 9, and 16, a short region on the long arm of chromosomes 1 and 2, and the distal heterochromatic part of the long arm of the Y chromosome were dispiralized. The despiralization pattern of DAPI is compared with those previously obtained with Hoechst 33258 and Distamycin A.

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页码：250 / 254

页数：5

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