DNA-SEQUENCE AND ANALYSIS OF THE BET GENES ENCODING THE OSMOREGULATORY CHOLINE GLYCINE BETAINE PATHWAY OF ESCHERICHIA-COLI

被引：221

作者：

LAMARK, T ^{[1
]}

KAASEN, I ^{[1
]}

ESHOO, MW ^{[1
]}

FALKENBERG, P ^{[1
]}

MCDOUGALL, J ^{[1
]}

STROM, AR ^{[1
]}

机构：

[1] UNIV TROMSO,NORWEGIAN COLL FISHERY SCI,DRAMSVEIEN 201B,N-9000 TROMSO,NORWAY

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb01877.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence was determined of 6493 nucleotides encompassing the bet genes of Escherichia coli which encode the osmoregulatory choline-glycine betaine pathway. Four open reading frames were identified: betA encoding choline dehydrogenase, a flavoprotein of 61.9 kDa; betB encoding betaine aldehyde dehydrogenase (52.8 kDa); betT encoding a proton-motive-force-driven, high-affinity transport system for choline (75.8 kDa); and betl, capable of encoding a protein of 21.8 kDa, implicated as a repressor involved in choline regulation of the bet genes. Identification of the genes was supported by subcloning, physical mapping of lambda placMu53 insertions, amino acid sequence similarity, or N-terminal amino acid sequencing. The bet genes are tightly spaced, with betT located upstream of, and transcribed divergently to, the tandemly linked betlBA genes.

引用

页码：1049 / 1064

页数：16

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