IMMUNOHISTOCHEMICAL ANALYSIS OF P53 AND HER-2/NEU PROTEINS IN HUMAN TUMORS

被引：82

作者：

CHANG, K ^{[1
]}

DING, I ^{[1
]}

KERN, FG ^{[1
]}

WILLINGHAM, MC ^{[1
]}

机构：

[1] GEORGETOWN UNIV,MED CTR,VINCENT T LOMBARDI CANC RES CTR,WASHINGTON,DC 20007

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1991年 / 39卷 / 09期

关键词：

P53; HER-2/NEU; IMMUNOHISTOCHEMISTRY; PEROXIDASE; HUMAN BREAST CANCER; COLON CANCER; OVARIAN CANCER;

D O I：

10.1177/39.9.1680897

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We examined samples of tumors of human breast, ovary, and colon of various degrees of malignancy for the expression of p53 protein, using a panel of anti-p53 antibodies and peroxidase immunohistochemistry. Of 66 tumor cases (24 cases of ovarian carcinoma, 23 cases of colon adenocarcinoma, and 19 cases of breast carcinoma), 36 (53%) showed high levels of expression of p53 using a human-specific antibody, and 16 (24%) showed high expression of a mutant form of p53. In the mutant p53-positive breast tumor samples, six (86%) were positive for HER-2/neu reactivity, compared with colon (0/4) and ovarian tumors (115). The pattern of p53 intracellular localization and tissue distribution, and the relationship between the expression of mutant p53 and cell differentiation, were also examined; poorly differentiated cells showed either overexpression of p53 of higher levels of mutant p53 in comparison with more normal cells.

引用

页码：1281 / 1287

页数：7

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