HB F-SASSARI - A NOVEL (G)GAMMA VARIANT WITH A THREONINE RESIDUE AT POSITION GAMMA-75, CHARACTERIZED BY MASS-SPECTROMETRIC TECHNIQUES

被引：10

作者：

FERRANTI, P

BARONE, F

PUCCI, P

MALORNI, A

MARINO, G

PILO, G

MANCA, L

MASALA, B

机构：

[1] Istituto di Industrie Agrarie, Universita di Napoli, 80100, Napoli

[2] Servizio di Spettrometria di Massa, 80131, Napoli

[3] Dipartimento di Chimica Organica e Biologica Universita di Napoli, 80100, Napoli

[4] Istituto di Chimica delle Molecole di Interesse, Biologico Consiglio Nazionale delle Ricerche, 80100, Napoli

[5] Centro per 1'Ingegneria Genetica e Biotecnologie Avanzate S.r.l., 80100, Napoli

[6] Istituto di Fisiologia Generale e Chimica Biologica Universita di Sassari, 07100, Sassari

来源：

HEMOGLOBIN | 1994年 / 18卷 / 4-5期

关键词：

D O I：

10.3109/03630269408996196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cord blood sample of a Caucasian newborn contained about 40% of an abnormal fetal hemoglobin. The mutated gamma chain was isolated using reversed phase high performance liquid chromatography and characterized by means of electrospray and fast atom bombardment mass spectrometric techniques as a (G) gamma-globin variant with an Ile->Thr substitution at position gamma 75. The variant chain shows the same structure as the previously described Hb F-Charlotte that was demonstrated to be an (A) gamma variant with an Ile->Thr substitution at position gamma 75 and an additional Ala->Gly substitution at gamma 136.

引用

页码：307 / 315

页数：9

共 17 条

[1] GLOBIN CHAIN ELECTROPHORESIS - A NEW APPROACH TO THE DETERMINATION OF THE G-GAMMA/A-GAMMA RATIO IN FETAL HEMOGLOBIN AND TO STUDIES OF GLOBIN-SYNTHESIS [J].