MICROBIOLOGICAL VALIDATION OF ASSESSMENTS OF CARIES ACTIVITY DURING CAVITY PREPARATION

被引:160
作者
KIDD, EAM
JOYSTONBECHAL, S
BEIGHTON, D
机构
[1] UNIV LONDON KINGS COLL,SCH MED & DENT,ROYAL COLL SURGEONS,DEPT DENT SCI,LONDON WC2R 2LS,ENGLAND
[2] UNIV LONDON LONDON HOSP,COLL MED,DEPT ORAL MED & PERIODONTOL,LONDON E1 1BB,ENGLAND
关键词
CARIES ACTIVITY; CAVITY PREPARATION; MICROBIOLOGY;
D O I
10.1159/000261571
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The operative management of primary and secondary caries assumes that all discoloured tissue at the enamel-dentine junction (EDJ) represents active disease and this is removed to arrest the carious process. This study aims to establish clinical criteria to differentiate between active and arrested caries at the EDJ using microbiological assessment of dentine samples to verify its clinical status. Radiographs were available for posterior teeth. Cavities (n = 205) were prepared under rubber dam. After gaining access, areas of the EDJ were chosen and assessments made of consistency (soft, medium, hard), colour (dark brown, mid-brown, pale) and moisture content (wet, dry). Dentine was removed by using a No. 3 round burr and placed in 1 ml of bacteriological culture broth. This sampling procedure was repeated at the same site once during cavity preparation and again when the cavity was judged as fully prepared. Samples were vortexed, diluted and cultured to give viable counts of the total anaerobic microflora, mutans streptococci and lactobacilli; viable counts were expressed as log10 (CFU per sample +1). Results showed no significant differences between the microflora of primary and secondary caries. The number of bacteria recovered diminished significantly as cavities were completed. Initial samples from soft and wet lesions harboured significantly more bacteria, lactobacilli and mutans streptococci than samples from medium, hard or dry lesions. Lesions visible on radiographs harboured more bacteria, including lactobacilli and mutans streptococci, while dentine colour was not discriminatory. In conclusion, the relevant clinical criteria for the diagnosis of infected dentine at the EDJ were visibility on radiographs, soft lesions and wet lesions.
引用
收藏
页码:402 / 408
页数:7
相关论文
共 15 条
[1]   A COMPARISON OF DIGITAL AND OPTICAL CRITERIA FOR DETECTING CARIOUS DENTIN [J].
ANDERSON, MH ;
CHARBENEAU, GT .
JOURNAL OF PROSTHETIC DENTISTRY, 1985, 53 (05) :643-646
[2]   SALIVARY LEVELS OF MUTANS STREPTOCOCCI, LACTOBACILLI, YEASTS, AND ROOT CARIES PREVALENCE IN NONINSTITUTIONALIZED ELDERLY DENTAL PATIENTS [J].
BEIGHTON, D ;
HELLYER, PH ;
LYNCH, EJR ;
HEATH, MR .
COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY, 1991, 19 (05) :302-307
[3]   A SIMPLE BIOCHEMICAL SCHEME FOR THE DIFFERENTIATION OF STREPTOCOCCUS-MUTANS AND STREPTOCOCCUS-SOBRINUS [J].
BEIGHTON, D ;
RUSSELL, RRB ;
WHILEY, RA .
CARIES RESEARCH, 1991, 25 (03) :174-178
[4]  
Charbeneau G.T., 1981, PRINCIPLES PRACTICE, V2nd ed., P208
[5]   SELECTIVE MEDIUM FOR STREPTOCOCCUS-MUTANS [J].
GOLD, OG ;
JORDAN, HV ;
VANHOUTE, J .
ARCHIVES OF ORAL BIOLOGY, 1973, 18 (11) :1357-1364
[6]   ROOT CARIES IN OLDER-PEOPLE ATTENDING A GENERAL DENTAL PRACTICE IN EAST-SUSSEX [J].
HELLYER, PH ;
BEIGHTON, D ;
HEATH, MR ;
LYNCH, EJR .
BRITISH DENTAL JOURNAL, 1990, 169 (07) :201-206
[7]  
Kidd E A, 1991, Int Dent J, V41, P279
[8]  
Kidd E A, 1990, Int Dent J, V40, P219
[9]   THE USE OF A CARIES DETECTOR DYE DURING CAVITY PREPARATION - A MICROBIOLOGICAL ASSESSMENT [J].
KIDD, EAM ;
JOYSTONBECHAL, S ;
BEIGHTON, D .
BRITISH DENTAL JOURNAL, 1993, 174 (07) :245-248
[10]   THE USE OF A CARIES DETECTOR DYE IN CAVITY PREPARATION [J].
KIDD, EAM ;
JOYSTONBECHAL, S ;
SMITH, MM ;
ALLAN, R ;
HOWE, L ;
SMITH, SR .
BRITISH DENTAL JOURNAL, 1989, 167 (04) :132-134