Establishment of immortal hepatocytes from a CYP3A7-transgenic p53-knockout mouse

被引：9

作者：

Shimoji, M

Hattori, K

Itoh, S

Nakayama, K

Katsuki, M

Aizawa, S

Yokoi, T

Kamataki, T

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,DIV DRUG METAB,SAPPORO,HOKKAIDO 060,JAPAN

[2] KYUSHU UNIV,MED INST BIOREGULAT,DEPT MOLEC CELL BIOL,HIGASHI KU,FUKUOKA 812,JAPAN

[3] KUMAMOTO UNIV,SCH MED,IMEG,MORPHOGENESIS LAB,KUMAMOTO 860,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2869

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mRNA, which is specifically expressed in the liver, remained in these cells. (C) 1995 academic Press, Inc.

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页码：1001 / 1005

页数：5

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