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AN IMPROVED PROCEDURE FOR DERIVATIZATION OF CONTROLLED-PORE GLASS-BEADS FOR SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS

被引：103
作者：
DAMHA, MJ
GIANNARIS, PA
ZABARYLO, SV
机构：
[1] Department of Chemistry, Erindale College, University of Toronto, Mississauga
来源：
NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 13期
基金：
加拿大自然科学与工程研究理事会;
关键词：
D O I：
10.1093/nar/18.13.3813
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-0-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required. © 1990 Oxford University Press.
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页码：3813 / 3821
页数：9
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