ALTERATION OF DNA-BINDING SPECIFICITY BY NICKEL-(II) SUBSTITUTION IN 3 ZINC-(II) FINGERS OF TRANSCRIPTION FACTOR SP1

被引：46

作者：

NAGAOKA, M ^{[1
]}

KUWAHARA, J ^{[1
]}

SUGIURA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,INST CHEM RES,UJI,KYOTO 611,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1996

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription factor Sp1, which has a DNA binding domain composed of three zinc fingers, binds to GC box (consensus sequence, G/T-GGGCGG-G/A-G/A-C/T) and activates the transcription by RNA polymerase II. Metal substitution of nickel(II) for zinc(II) in Sp1 causes no differences in the mode of protein-DNA interaction. However, sequence preference of Ni(II)Sp1 changes from 5’-GGGGCGGGGC to 5’-GGGGCGTGGC, and is distinct from that of Zn(II)Sp1. The result indicates an important effect of metal-induced folding on sequence-specific recognition of DNA by zinc-finger proteins. © 1993 Academic Press, Inc.

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页码：1515 / 1520

页数：6

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