EFFECTS OF FATTY-ACID OXIDATION ON GLUCOSE-UTILIZATION BY ISOLATED HEPATOCYTES

被引:25
作者
BERRY, MN [1 ]
PHILLIPS, JW [1 ]
HENLY, DC [1 ]
CLARK, DG [1 ]
机构
[1] CSIRO, DIV HUMAN NUTR, ADELAIDE, SA 5000, AUSTRALIA
关键词
GLUCOSE UTILIZATION; GLUCOSE FATTY ACID CYCLE; GLUCOSE GLUCOSE 6-PHOSPHATE FUTILE CYCLE; ISOLATED HEPATOCYTE; GLYCOLYSIS;
D O I
10.1016/0014-5793(93)80030-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have studied the inhibitory action of long- and short-chain fatty acids on hepatic glucose utilization in hepatocytes isolated from fasted rats. The rates of hepatic glucose phosphorylation and glycolysis were determined from the tritiated products of [2-H-3] and 16-H-3]glucose metabolism, respectively. The difference between these was taken as an estimate of the 'cycling' between glucose and glucose-6-phosphate. In the presence of 40 mM glucose this cycling was estimated at 0.68 mumol/min/g wet wt. Glucose phosphorylation was unaffected during palmitate and hexanoate oxidation to ketone bodies but glycolysis was inhibited. The rate of glucose cycling was increased during this phase to 1.25 mumol/min/g. Following the complete metabolism of the fatty acids, glycolysis was reinstated and cycling rates returned to control levels. Hepatic glucose cycling appears to be an important component of the glucose/fatty acid cycle.
引用
收藏
页码:26 / 30
页数:5
相关论文
共 44 条
[1]  
Bergmeyer HI., 1974, METHODS ENZYMATIC AN
[2]  
Berry M, 1991, ISOLATED HEPATOCYTES
[3]   EFFECTS OF BICARBONATE ON INTERCOMPARTMENTAL REDUCING-EQUIVALENT TRANSLOCATION IN ISOLATED PARENCHYMAL-CELLS FROM RAT-LIVER [J].
BERRY, MN ;
WERNER, HV ;
KUN, E .
BIOCHEMISTRY, 1974, 140 (03) :355-361
[4]   HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY [J].
BERRY, MN ;
FRIEND, DS .
JOURNAL OF CELL BIOLOGY, 1969, 43 (03) :506-+
[5]   THE CALORIGENIC NATURE OF HEPATIC KETOGENESIS - AN EXPLANATION FOR THE STIMULATION OF RESPIRATION INDUCED BY FATTY-ACID SUBSTRATES [J].
BERRY, MN ;
CLARK, DG ;
GRIVELL, AR ;
WALLACE, PG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 131 (01) :205-214
[6]  
BERRY MN, 1991, REGULATION HEPATIC F, P179
[7]   PHOSPHORYLATION OF GLUCOSE IN ISOLATED RAT HEPATOCYTES - SIGMOIDAL KINETICS EXPLAINED BY ACTIVITY OF GLUCOKINASE ALONE [J].
BONTEMPS, F ;
HUE, L ;
HERS, HG .
BIOCHEMICAL JOURNAL, 1978, 174 (02) :603-611
[8]   THE MOLECULAR-BASIS OF THE TYPE-1 GLYCOGEN-STORAGE DISEASES [J].
BURCHELL, A .
BIOESSAYS, 1992, 14 (06) :395-400
[9]  
CHEN RF, 1967, J BIOL CHEM, V242, P173
[10]   SUB-COMPARTMENTATION OF THE CYTOSOLIC GLUCOSE-6-PHOSPHATE POOL IN CULTURED RAT HEPATOCYTES [J].
CHRIST, B ;
JUNGERMANN, K .
FEBS LETTERS, 1987, 221 (02) :375-380