NEW METHOD FOR REMOVING NON-IONIC DETERGENT THAT ALLOWS RECONSTITUTION OF DOPAMINE-SENSITIVE ADENYLATE-CYCLASE

被引：11

作者：

HOFFMANN, FM

机构：

[1] Section of Biochemistry, Molecular and Cell Biology Cornell University, Ithaca

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)90215-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The macromolecular components of dopamine-sensitive adenylate cyclase are soluble in solutions of the nonionic detergents Brij 56 or digitonin, however response of the adenylate cyclase to dopamine is lost. Removal of the nonionic detergent by replacement with cholate and phospholipid, followed by removal of the cholate, restored the dopamine sensitivity of adenylate cyclase. By this method, the functional complex was reassembled from two separate solutions of components, one deficient in adenylate cyclase activity, and the other unresponsive to dopamine. © 1979.

引用

页码：988 / 994

页数：7

共 12 条

[1] HAGA T, 1977, J BIOL CHEM, V252, P5776
[2] HOFFMANN FM, 1979, THESIS CORNELL U
[3] HOFFMANN FM, J BIOL CHEM
[4] AFFINITY PURIFICATION OF CARDIAC ADENYLATE-CYCLASE - DEPENDENCE ON PRIOR HYDROPHOBIC RESOLUTION
HOMCY, C
WRENN, S
HABER, E
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (01) : 59 - 63
[5] KAGAWA Y, 1971, J BIOL CHEM, V246, P5477
[6] Levey G S, 1975, Adv Cyclic Nucleotide Res, V5, P53
[7] LIMBIRD LE, 1977, J BIOL CHEM, V252, P799
[8] NEER EJ, 1973, J BIOL CHEM, V248, P3742
[9] COUPLING OF CATECHOLAMINE RECEPTOR FROM ONE CELL WITH ADENYLATE-CYCLASE FROM ANOTHER CELL BY CELL-FUSION
ORLY, J
SCHRAMM, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (12) : 4410 - 4414
[10] PFEUFFER T, 1977, J BIOL CHEM, V252, P7224

← 1 2 →