THE 23S/5S RIBOSOMAL-RNA GENES (RRL/RRF) ARE SEPARATE FROM THE 16S RIBOSOMAL-RNA GENE (RRS) IN BORRELIA-BURGDORFERI, THE ETIOLOGIC AGENT OF LYME-DISEASE

被引：41

作者：

FUKUNAGA, M ^{[1
]}

YANAGIHARA, Y ^{[1
]}

SOHNAKA, M ^{[1
]}

机构：

[1] UNIV SHIZUOKA,SCH PHARMACEUT SCI,DEPT MICROBIOL,YADA,SHIZUOKA 422,JAPAN

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷

关键词：

D O I：

10.1099/00221287-138-5-871

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA fragments containing the rRNA genes for Borrelia burgdorferi strain B31 were cloned in bacteriophage-lambda-EMBL3. A restriction map of the fragments was constructed and the organization of the rRNA genes was determined by Southern hybridization. One genomic DNA fragment contained a single copy of the rrs sequence and another cloned fragment contained both rrl and rrf sequences. The results revealed that the rrs gene is located separately from the set of rrl/rrf genes, suggesting that these rRNA genes are expressed independently in B. burgdorferi.

引用

页码：871 / 877

页数：7

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