AUTOMATED LANES DETECTION AND COMPARISON OF BACTERIAL ELECTROPHORETIC PROTEIN FINGERPRINTS USING FAST FOURIER TRANSFORMATION

被引：4

作者：

MILLERSHIP, S

RAGOONADEN, K

机构：

[1] Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, W12 ONN, Du Cane Road

来源：

COMPUTERS AND BIOMEDICAL RESEARCH | 1992年 / 25卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0010-4809(92)90029-A

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

A method of computer-automated analysis of bacterial fingerprints produced by electrophoresis of proteins in a one-dimensional slab gel system is described. Proteins were visualized by silver staining, Western blotting, or autoradiography. Gels were recorded with a CCD camera, and after initial manual removal of the unwanted image margins, track margins were identified and extracted and a normalized trace was produced automatically using Fourier routines to smooth plots required for this process. Normalized traces were then compared by Fourier correlation after application of a high-pass step filter. © 1992.

引用

页码：392 / 406

页数：15

共 12 条

[1] MEASURES OF THE AMOUNT OF ECOLOGIC ASSOCIATION BETWEEN SPECIES [J].

DICE, LR .

ECOLOGY, 1945, 26 (03) :297-302

[2] QUANTITATIVE COMPARISON OF ELECTROPHORETIC TRACES OF BACTERIAL PROTEINS [J].

FELTHAM, RKA ;

SNEATH, PHA .

COMPUTERS AND BIOMEDICAL RESEARCH, 1979, 12 (03) :247-263

[3]

KERSTERS K, 1980, MICROBIAL CLASSIFICA

[4]

KERSTERS K, 1985, COMPUTER ASSISTED BA

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] TYPING OF AEROMONAS SPECIES BY PROTEIN FINGERPRINTING - COMPARISON OF RADIOLABELLING AND SILVER STAINING FOR VISUALIZING PROTEINS [J].

MILLERSHIP, SE ;

WANT, SV .

JOURNAL OF MEDICAL MICROBIOLOGY, 1989, 29 (01) :29-32

[7]

Press W.H., 1994, NUMERICAL RECIPES C, V2nd ed.

[8] COMPUTER AUTOMATED LANES DETECTION AND PROFILES EVALUATION OF ONE-DIMENSIONAL GEL-ELECTROPHORETIC AUTORADIOGRAMS [J].

PUN, T ;

TRUS, B ;

GROSSMAN, N ;

LEIVE, L ;

EDEN, M .

ELECTROPHORESIS, 1985, 6 (06) :268-274

[9] NEW METHOD FOR TYPING STAPHYLOCOCCUS-AUREUS RESISTANT TO METHICILLIN BASED ON S-35 METHIONINE LABELED PROTEINS - ITS APPLICATION IN AN OUTBREAK [J].

STEPHENSON, JR ;

CROOK, SJ ;

TABAQCHALI, S .

BRITISH MEDICAL JOURNAL, 1986, 293 (6547) :581-583

[10]

STEPHENSON JR, 1987, J MED MICROBIOL, V23, P113

← 1 2 →