A MUTATION IN RIBOSOMAL PROTEIN-L9 AFFECTS RIBOSOMAL HOPPING DURING TRANSLATION OF GENE-60 FROM BACTERIOPHAGE-T4

被引：43

作者：

HERBST, KL ^{[1
]}

NICHOLS, LM ^{[1
]}

GESTELAND, RF ^{[1
]}

WEISS, RB ^{[1
]}

机构：

[1] UNIV UTAH, DEPT HUMAN GENET, SALT LAKE CITY, UT 84112 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 26期

关键词：

D O I：

10.1073/pnas.91.26.12525

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ribosomes hop over a 50-nt coding gap during translation of gene 60 mRNA from bacteriophage T4. This event occurs with near-unitary efficiency when gene 60-lacZ fusions are expressed in Escherichia coli. One of the components necessary for this hop is an RNA hairpin structure containing the 5' junction of the 50-nt coding gap. A mutant E. coli was isolated and found to significantly increase hopping when carrying gene 60-lacZ constructs with altered hairpins. The mutation, hop-1, changed Ser(93) to Phe in rplI, the gene coding for ribosomal large-subunit protein L9. Ribosomal hopping on a synthetic sequence in the absence of a hairpin was also increased by this mutation. These data suggest that hop-1 may substitute for the function of the hairpin during ribosomal hopping.

引用

页码：12525 / 12529

页数：5

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