INTERRUPTION OF THE FERREDOXIN (FLAVODOXIN) NADP(+) OXIDOREDUCTASE GENE OF ESCHERICHIA-COLI DOES NOT AFFECT ANAEROBIC GROWTH BUT INCREASES SENSITIVITY TO PARAQUAT

被引：48

作者：

BIANCHI, V ^{[1
]}

HAGGARDLJUNGQUIST, E ^{[1
]}

PONTIS, E ^{[1
]}

REICHARD, P ^{[1
]}

机构：

[1] KAROLINSKA INST, MBB, MED NOBEL INST, DEPT BIOCHEM 1, S-17177 STOCKHOLM, SWEDEN

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 15期

关键词：

D O I：

10.1128/jb.177.15.4528-4531.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ferredoxin (flavodoxin) NADP(+) oxidoreductase participates in methionine biosynthesis and in the function of two anaerobic enzymes, pyruvate formate-lyase and ribonucleotide reductase. We prepared insertion mutants of Escherichia coli lacking a functional enzyme. They do not require methionine and they grow well anaerobically, but they show increased sensitivity to paraquat.

引用

页码：4528 / 4531

页数：4

共 22 条

[1] THE HEMOGLOBIN-LIKE PROTEIN (HMP) OF ESCHERICHIA-COLI HAS FERRISIDEROPHORE REDUCTASE-ACTIVITY AND ITS C-TERMINAL DOMAIN SHARES HOMOLOGY WITH FERREDOXIN NADP+ REDUCTASES [J].

ANDREWS, SC ;

SHIPLEY, D ;

KEEN, JN ;

FINDLAY, JBC ;

HARRISON, PM ;

GUEST, JR .

FEBS LETTERS, 1992, 302 (03) :247-252

[2] MECHANISM OF REDUCTIVE ACTIVATION OF COBALAMIN-DEPENDENT METHIONINE SYNTHASE - AN ELECTRON-PARAMAGNETIC RESONANCE SPECTROELECTROCHEMICAL STUDY [J].

BANERJEE, RV ;

HARDER, SR ;

RAGSDALE, SW ;

MATTHEWS, RG .

BIOCHEMISTRY, 1990, 29 (05) :1129-1135

[3] ESCHERICHIA-COLI FERREDOXIN NADP+ REDUCTASE - ACTIVATION OF ESCHERICHIA-COLI ANAEROBIC RIBONUCLEOTIDE REDUCTION, CLONING OF THE GENE (FPR), AND OVEREXPRESSION OF THE PROTEIN [J].

BIANCHI, V ;

REICHARD, P ;

ELIASSON, R ;

PONTIS, E ;

KROOK, M ;

JORNVALL, H ;

HAGGARDLJUNGOUIST, E .

JOURNAL OF BACTERIOLOGY, 1993, 175 (06) :1590-1595

[4] FLAVODOXIN IS REQUIRED FOR THE ACTIVATION OF THE ANAEROBIC RIBONUCLEOTIDE REDUCTASE [J].

BIANCHI, V ;

ELIASSON, R ;

FONTECAVE, M ;

MULLIEZ, E ;

HOOVER, DM ;

MATTHEWS, RG ;

REICHARD, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 197 (02) :792-797

[5]

BLASCHKOWSKI HP, 1982, EUR J BIOCHEM, V123, P563

[6] MOLECULAR-CLONING, SEQUENCE AND REGULATION OF EXPRESSION OF THE RECA GENE OF THE PHOTOTROPHIC BACTERIUM RHODOBACTER-SPHAEROIDES [J].

CALERO, S ;

DEHENESTROSA, ARF ;

BARBE, J .

MOLECULAR & GENERAL GENETICS, 1994, 242 (01) :116-120

[7] MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].

DELORENZO, V ;

HERRERO, M ;

JAKUBZIK, U ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572

[8] REDOX REDUX - THE CONTROL OF OXIDATIVE STRESS RESPONSES [J].

DEMPLE, B ;

AMABILECUEVAS, CF .

CELL, 1991, 67 (05) :837-839

[9]

ELIASSON R, 1992, J BIOL CHEM, V267, P25541

[10]

FUJII K, 1974, J BIOL CHEM, V249, P6745

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