UP-REGULATION OF ANTITHROMBOTIC ECTONUCLEOTIDASES BY ASPIRIN IN HUMAN ENDOTHELIAL-CELLS IN-VITRO

被引：5

作者：

CHEUNG, PK ^{[1
]}

VISSER, J ^{[1
]}

BAKKER, WW ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT THERAPEUT & PHARMACOL,9713 EZ GRONINGEN,NETHERLANDS

来源：

JOURNAL OF PHARMACY AND PHARMACOLOGY | 1994年 / 46卷 / 12期

关键词：

D O I：

10.1111/j.2042-7158.1994.tb03262.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Ecto ATP-diphosphohydrolase (apyrase) activity of human endothelial cells following aspirin treatment has been studied in-vitro. It was shown by HPLC analysis of supernatant samples that pre-incubation of the cultures with aspirin resulted in a significantly increased turnover of supplemented ATP into its degradation products (ADP and AMP). Enhanced expression of ectoenzyme after aspirin treatment could be observed as demonstrated by immunofluorescence-staining with monoclonal anti-apyrase antibodies. This suggests enhancement of endothelial ATP-diphosphohydrolase activity induced by aspirin. The present data obtained in human vascular cells in-vitro are in line with results from previous animal studies in-vivo, suggesting a novel cycleoxygenase-independent antithrombotic activity of aspirin.

引用

页码：1032 / 1034

页数：3

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