COMPARATIVE-STUDY ON HYDROLYSIS OF TRIOLEIN IN REVERSED MICELLAR SYSTEM CATALYZED BY DIFFERENT TYPES OF LIPASES

Hydrolysis of triolein in AOT/isooctane reversed micelles by an sn-1,3-regioselective and a non-selective lipase were studied. Kinetics of the multistep reaction: decomposition of tri-, di- and monoacylglycerols and production of fatty acid were investigated separately. All the reactions was found to obey the Michaelis-Menten model and the apparent parameters (Michaelis-constants (K-m) and maximal reaction rates (V-max)) were determined both for non-selective and regioselective preparations.