SPHEROPLAST FUSION OF BREWERS-YEAST STRAINS

被引：45

作者：

RUSSELL, I

STEWART, GG

机构：

[1] Brewing Research and Development Department, Labatt Breweries of Canada Ltd., London, Ontario

来源：

JOURNAL OF THE INSTITUTE OF BREWING | 1979年 / 85卷 / 02期

关键词：

hybridization; protoplast; Saccharomyces; sexuality; yeast;

D O I：

10.1002/j.2050-0416.1979.tb06834.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Spheroplasts of brewing polyploid yeast strains have been successfully fused with spheroplasts of haploid yeast strains. After regeneration of the cell wall, stable fusion recombinants were isolated. Genetic analysis of these recombinants revealed that they contained the genotype of both parents, sporulated well with each ascus containing four spores and were indeed diploid. Spheroplast fusion thus affords a means to genetically analyse brewing yeast strains, such an analysis having been difficult if not impossible by conventional hybridization techniques. 1979 The Institute of Brewing & Distilling

引用

页码：95 / 98

页数：4

共 41 条

[1] SPORULATION AND MATING OF BREWING YEASTS [J].