VERSATILE INSTRUMENTATION FOR ONE-DIMENSIONAL AND 2-DIMENSIONAL MICROSCALE POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：6

作者：

ANSELSTETTER, V ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG, INST PHYSIOL CHEM, D-8520 ERLANGEN, FED REP GER

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 172卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(00)90944-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An all-glass electrophoresis cell for slab gels was developed. It has excellent separation qualities in polyacrylamide gel electrophoresis. Instrumentation is presented for the use of this cell with various techniques of 1- and 2-dimensional electrophoresis. Special attention is paid to gradient gel electrophoresis. The features of the instrumentation are discussed in detail and examples [separation of serum proteins, Ehrlich ascites cell proteins, trypsin inhibitor, cytochrome c, aprotinin, erythrocyte membrane proteins and bronchial mucus glycoproteins] are given to demonstrate the quality of the electrophoretic separation.

引用

页码：49 / 56

页数：8

共 11 条

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