THERMODYNAMIC STUDY OF LANGMUIR ANTIBODY FILMS FOR APPLICATION TO IMMUNOSENSORS

被引：37

作者：

AHLUWALIA, A ^{[1
]}

DEROSSI, D ^{[1
]}

MONICI, M ^{[1
]}

SCHIRONE, A ^{[1
]}

机构：

[1] ENTE NAZL ENERGIA ATOM,CTR RIC ENERGIA AMBIENTE,I-19100 LA SPEZIA,ITALY

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 02期

关键词：

LANGMUIR-BLODGETT FILMS; IMMUNOGLOBULIN-G; TOTAL INTERNAL REFLECTION FLUORESCENCE; IMMUNOSENSOR;

D O I：

10.1016/0956-5663(91)87037-C

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The thermodynamic functions, compressibility, entropy and free energy of desorption have been derived from a set of pi-A isotherms of human immunoglobulin G films at an air-water interface. Criteria for optimum filmlift conditions have been obtained from an analysis of these functions. By the application of a basic model, a picture of the desorption behaviour of segments of the antibodies has also been constructed. The results of this study have been used to estimate the theoretical sensing limit of a TIRF based immunosensor.

引用

页码：133 / 141

页数：9

共 23 条

[1] AHLUWALIA A, 1990, UNPUB BIOSENSORS BIO
[2] OPTICAL-PROPERTIES OF THIN-LAYERS OF BOVINE SERUM-ALBUMIN, GAMMA-GLOBULIN, AND HEMOGLOBIN
ARWIN, H
[J]. APPLIED SPECTROSCOPY, 1986, 40 (03) : 313 - 318
[3] DAVIES JT, 1961, INTERFACIAL PHENOMEN, P248
[4] EDDOWES MJ, 1987, BIOSENSORS, V3, P1
[5] THERMODYNAMIC AND RHEOLOGICAL PROPERTIES OF MACROMOLECULAR MONOLAYERS
GABRIELLI, G
PUGGELLI, M
FERRONI, E
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1971, 36 (03) : 401 - +
[6] GAINS GL, 1966, INSOLUBLE MONOLAYERS, P271
[7] GORTER E, 1973, T FARADAY SOC, V33, P1125
[8] PROTEIN ADSORPTION ON THE SURFACE OF A THIN-FILM POLYMER INTEGRATED OPTICAL WAVE-GUIDE
IVES, JT
REICHERT, WM
[J]. APPLIED SPECTROSCOPY, 1988, 42 (01) : 68 - 72
[9] NEW IMMUNOASSAY BASED ON FLUORESCENCE EXCITATION BY INTERNAL-REFLECTION SPECTROSCOPY
KRONICK, MN
LITTLE, WA
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1975, 8 (03) : 235 - 240
[10] ANGULAR-DISTRIBUTION OF FLUORESCENCE FROM LIQUIDS AND MONODISPERSED SPHERES BY EVANESCENT WAVE EXCITATION
LEE, EH
BENNER, RE
FENN, JB
CHANG, RK
[J]. APPLIED OPTICS, 1979, 18 (06): : 862 - 868

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