PURIFICATION AND CRYSTALLIZATION OF FUMARASE-C FROM ESCHERICHIA-COLI

Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C2221 form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C2221 form is complete to 2.10 Å and the I222 form is complete to 2.20 Å. © 1993 Academic Press, Inc.