Application of capillary electrophoresis in the identification of phenotypes containing the beta-lactoglobulin C variant

被引:27
作者
Paterson, GR
Otter, DE
Hill, JP
机构
[1] Food Science Section, New Zealand Dairy Research Institute, Palrnerston North
关键词
beta-lactoglobulin; phenotypes; milk protein variants; capillary electrophoresis;
D O I
10.3168/jds.S0022-0302(95)76893-X
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
A method for the separation of the beta-LG variants A, B, and C was developed using free zone capillary electrophoresis. A separation buffer consisting of 50 mM 2-(N-morpholino)ethane sulfonic acid (pH 8.0) and polyoxyethylene (20)-sorbitan monolaurate was used for the separation. Milk samples from 424 Jersey cows were phenotyped using this method, and the gene frequencies for beta-LG A, B, and C were .41, .53, and .06, respectively. These frequencies were different from those found in studies of Jersey populations in other countries, where the beta-LG B allele had a significantly higher frequency, and the beta-LG A allele a significantly lower frequency, than those of this present study. The frequency of the beta-LG C allele was intermediate to those of other studies. The concentration of beta-LG in the milk was different among the beta-LG phenotypes and was, from greatest to least: AA, AB, AC, BE, and BC.
引用
收藏
页码:2637 / 2644
页数:8
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