POLYADENYLYLATION HELPS REGULATE MESSENGER-RNA DECAY IN ESCHERICHIA-COLI

被引：210

作者：

OHARA, EB ^{[1
]}

CHEKANOVA, JA ^{[1
]}

INGLE, CA ^{[1
]}

KUSHNER, ZR ^{[1
]}

PETERS, E ^{[1
]}

KUSHNER, SR ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT GENET,ATHENS,GA 30602

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 06期

关键词：

POLY(A) POLYMERASE; TRXA; OMPA; LPP; MESSENGER-RNA HALF-LIVES;

D O I：

10.1073/pnas.92.6.1807

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

As part of our genetic analysis of mRNA decay in Escherichia coli K-12, we examined the effect of the pcnB gene [encoding poly(A) polymerase I] on message stability. Eliminating poly(A) polymerase I (Delta pcnB) dramatically stabilized the lpp, ompA, and trxA transcripts. The half lives of individual mRNAs were increased in both a Delta pcnB single mutant and a Delta pcnB pnp-7 rnb-500 rne-1 multiple mutant. We also found mRNA decay intermediates in Delta pcnB mutants that were not detected in control strains, By end-labeling total E. coli RNA with [P-32]pCp and T4 RNA ligase and then digesting the RNA with RNase A and T-1, we showed that many RNAs in a wild-type strain contained poly(A) tails ranging from 10 nt to >50 nt long. When polynucleotide phosphorylase, RNase II, and RNase E were absent, the length (>100 nt) and number (10- to 20-fold) of the poly(A) tails increased. After transcription initiation was stopped with rifampicin, polyadenylylation apparently continued. Deleting the structural gene for poly(A) polymerase I (pcnB) reduced the amount of 3'-terminal poly(A) sequences by >90%. We propose a model for the role of polyadenylylation in mRNA decay,

引用

页码：1807 / 1811

页数：5

共 33 条

[1] DETERMINATION OF THE LENGTH DISTRIBUTION OF POLY(A) AT THE 3' TERMINUS OF THE VIRION RNAS OF EMC VIRUS, POLIOVIRUS, RHINOVIRUS, RAV-61 AND CPMV AND OF MOUSE GLOBIN MESSENGER-RNA [J].