THE INVOLVEMENT OF FRUCTOSE 2,6-BISPHOSPHATE IN SUBSTRATE CYCLE CONTROL IN THE NONOXIDATIVE STAGE OF THE PENTOSE-PHOSPHATE PATHWAY - A PHOSPHORUS MAGNETIC-RESONANCE SPECTROSCOPY STUDY

被引：4

作者：

BELYAEVA, NF

GOLUBEV, MA

GRIGOROVICH, JA

DUBINSKY, ZV

SEMENOVA, NA

PITKANEN, E

KOROVKIN, BF

机构：

[1] RUSSIAN ACAD SCI,INST CHEM PHYS,MOSCOW,RUSSIA

[2] HELSINKI UNIV HOSP,DEPT MED 4,HELSINKI,FINLAND

来源：

EXPERIENTIA | 1994年 / 50卷 / 08期

关键词：

FRUCTOSE 2,6-BISPHOSPHATE; NMR-SPECTROSCOPY; 6-PHOSPHOFRUCTO-1-KINASE; RAT LIVER; SEDOHEPTULOSE; 7-PHOSPHATE; SEDOHEPTULOSE 1,7-BISPHOSPHATE;

D O I：

10.1007/BF01919382

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The role of fructose 2,6-bisphosphate in the interconversion of sedoheptulose 7-phosphate and sedoheptulose 1,7-bisphosphate in rat liver cytosol fractions was studied by means of phosphorus magnetic resonance spectroscopy. When the activity of 6-phosphofructo-1-kinase was inhibited by a high concentration of ATP, the addition of fructose 2,6-bisphosphate led to a marked decrease in sedoheptulose 7-phosphate levels, accompanied by an increased concentration of ADP. Fructose 2,6-bisphosphate essentially inhibited both the decrease in sedoheptulose 1,7-bisphosphate concentration and the accumulation of P-i in the incubation mixture. The data provided evidence that fructose 2,6-bisphosphate can regulate the substrate cycle: sedoheptulose 7-phosphate reversible arrow sedoheptulose 1,7-bisphosphate in the liver, and thus control the flux through the nonoxidative stage of the pentose phosphate pathway.

引用

页码：780 / 784

页数：5

共 18 条

[1]

BELYAEVA NF, 1989, BIOCHEMISTRY-MOSCOW+, V54, P1236

[2] REGULATION OF HEPATIC ALTRO HEPTULOSE 1,7-BISPHOSPHATE LEVELS AND CONTROL OF FLUX THROUGH THE PENTOSE PATHWAY BY FRUCTOSE 2,6-BISPHOSPHATE [J].