HIGH-LEVEL EXPRESSION OF TISSUE INHIBITOR OF METALLOPROTEINASES IN CHINESE HAMSTER OVARY CELLS USING GLUTAMINE-SYNTHETASE GENE AMPLIFICATION

被引：224

作者：

COCKETT, MI

BEBBINGTON, CR

YARRANTON, GT

机构：

[1] Celltech Limited, Berkshire, SL1 4EN

来源：

BIO-TECHNOLOGY | 1990年 / 8卷 / 07期

关键词：

D O I：

10.1038/nbt0790-662

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used a glutamine synthetase (GS) gene as an amplifiable marker in Chinese hamster ovary (CHO) cells. GS was combined with an efficient transcription unit to produce tissue inhibitor of metallopro- teinases (TIMP). Initial transfectant celllines selected using a GS gene secreted up to 9jxg TIMP/10" cells/24h. After one round of GS gene amplification expression levels of llOpg TIMP/1 O'5 cells/24h were achieved. These GS gene amplified CHO cells, when adapted to grow in suspension, accumulated 180mg/l in shake flask culture. This system therefore provides a rapid method of achieving high level gene expression in mammalian cells. © 1990 Nature Publishing Group.

引用

页码：662 / 667

页数：6

共 32 条

[1]

Bebbington CR, 1987, DNA CLONING PRACTICA, VIII, P163

[2]

BIRCH JR, 1985, TRENDS BIOTECHNOL, V3, P162, DOI 10.1016/0167-7799(85)90115-5

[3] USE OF THE ESCHERICHIA-COLI GENE FOR ASPARAGINE SYNTHETASE AS A SELECTIVE MARKER IN A SHUTTLE VECTOR CAPABLE OF DOMINANT TRANSFECTION AND AMPLIFICATION IN ANIMAL-CELLS [J].