CORRECTION OF CD18-DEFICIENT LYMPHOCYTES BY RETROVIRUS-MEDIATED GENE-TRANSFER

被引：61

作者：

WILSON, JM

PING, AJ

KRAUSS, JC

MAYOBOND, L

ROGERS, CE

ANDERSON, DC

TODD, RF

机构：

[1] TEXAS CHILDRENS HOSP,SPEROS P MARTEL LEUKOCYTE BIOL LAB,HOUSTON,TX 77030

[2] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48109

[3] UNIV MICHIGAN,SCH MED,PROGRAM CELLULAR & MOLEC BIOL,ANN ARBOR,MI 48109

[4] BAYLOR UNIV,DEPT PEDIAT,HOUSTON,TX 77030

[5] BAYLOR UNIV,DEPT CELL BIOL,HOUSTON,TX 77030

来源：

SCIENCE | 1990年 / 248卷 / 4961期

关键词：

D O I：

10.1126/science.1972597

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function caused by derangements in CD18 expression. The genetic and functional abnormalities in a lymphocyte cell line from a patient with LAD have been corrected by retrovirus-mediated transduction of a functional CD18 gene. Lymphocytes from patients with LAD were exposed to CD18-expressing retrovirus and enriched for cells that express CD11a and CD18 (LFA-1) on the cell surface. Molecular and functional analyses of these cells revealed (i) one copy of proviral sequence per cell, (ii) viral-directed CD18 RNA that exceeded normal endogenous levels, (iii) normal quantities of CD11a and CD18 protein on the cell surface, and (iv) reconstitution of LFA-1-dependent adhesive function.

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页码：1413 / 1416

页数：4

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