ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETERMINATION OF IGM RHEUMATOID-FACTOR

被引：33

作者：

VEJTORP, M

HOIERMADSEN, M

HALBERG, P

机构：

[1] STATENS SERUM INST,DEPT BLOOD GROUPING,DK-2300 COPENHAGEN S,DENMARK

[2] STATENS SERUM INST,BLOOD BANK,DK-2300 COPENHAGEN S,DENMARK

[3] UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT MED,DIV RHEUMATOL,DK-2650 HVIDOVRE,DENMARK

来源：

SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1979年 / 8卷 / 02期

关键词：

D O I：

10.3109/03009747909105338

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A semi-automated enzyme-linked immunosorbent assay (ELISA) for determination of IgM rheumatoid factor was established. Human gammaglobulin (Cohn fraction II) was used as an antigen on a solid phase of polystyrene microtitre trays. The results were read on a spectrophotometer. ELISA and the sheep cell agglutination test were compared in a study of sera from 400 blood donors, 53 patients with active rheumatoid arthritis and of 200 sera received for routine determination of rheumatoid factor. The results of the two tests correlated well. The ELISA procedure was precise, objective, inexpensive and well suited for quantitative routine determination of IgM rheumatoid factor. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：65 / 70

页数：6

共 22 条

[1]

ANDERSON SG, 1970, B WORLD HEALTH ORGAN, V42, P311

[2] ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) - MEASURE OF ANTIBODY CONCENTRATION OR AFFINITY [J].