NEUROTENSIN-LIKE IMMUNOREACTIVITY IN THE DIENCEPHALON OF THE ADULT MALE CAT

Using an indirect immunoperoxidase technique, the location of neurotensin-like fibers and cell bodies was studied in the diencephalon of the cat. The findings showed that the hypothalamus is richer in neurotensin-like-immunoreactive structures than the thalamus, and that neurotensin-like-immunoreactive structures are more widely distributed in the hypothalamus than in the thalamus. A high density of immunoreactive fibers was observed in the hypothalamic regions, area hypothalamica dorsalis, hypothalamus posterior, nucleus (n.) filiformis and n. arcuatus, whereas a moderate density was found in the n. parafascicularis, n. paraventricularis anterior, hypothalamus lateralis, median eminence and n. paraventricularis hypothalami. Other diencephalic regions such as n. lateralis posterior, n. lateralis dorsalis, n. medialis dorsalis, n. habenularis lateralis, n. centrum medianum, n. rhomboidens, n. reuniens, hypothalamus anterior, n. supra chiasmaticus, hypothalamus ventromedialis, n. supraopticus and hypothalamus dorsomedialis had the lowest density of immunoreactive fibers. In addition, the densest clusters of neurotensin-like perikarya were found in the n. arcuatus, n. centralis medialis and hypothalamus posterior, whereas the n. medialis dorsalis, n. paraventricularis anterior, n. reuniens, hypothalamus lateralis and hypothalamus ventromedialis had the lowest density. In the n. lateralis dorsalis, n. supraopticus, area hypothalamica dorsalis and n. supra chiasmaticus the density of immunoreactive perikarya was moderate.