COMPARISON OF SUBSTRATE UTILIZATION AND GROWTH-KINETICS BETWEEN IMMOBILIZED AND SUSPENDED PSEUDOMONAS CELLS

A methodology is described for measurement of immobilized and suspended cell growth and substrate utilization kinetic parameters. Substrate utilization and growth kinetics were compared between immobilized and suspended cells for toluene degrading Pseudomonas strain K3-2 and 2,4-dichlorophenoxyacetic acid (2,4-D) degrading strain DBO131(pRO101), respectively. kinetic parameters were estimated using nonlinear parameter estimation methods and compared between the immobilized and suspended Pseudomonas cells to determine the effect of immobilization on cellular growth and substrate utilization. Factors influencing the experimental design included calculated oxygen flux rates, primary carbon substrate flux rates, and shear stresses on the immobilized cell. Statistical interpretation of the cellular reaction rate parameters indicates that only the growth kinetics of the toluene system were significantly altered upon immobilization. Substrate utilization kinetics remained unchanged upon immobilization. The substrate growth associated half-saturation constant (K(g)) for the toluene system increased by 30-fold and the maximum specific growth rate (mu(max)) decreased by 2-fold upon immobilization. Implications of these results for experimental determination of cellular kinetic parameters and for immobilized cell bioreactor design are discussed.