BACTERIOPHAGE PHI-KP MEDIATED GENERALIZED TRANSDUCTION IN ERWINIA-CAROTOVORA SUBSPECIES CAROTOVORA

被引：27

作者：

TOTH, I

PEROMBELON, M

SALMOND, G

机构：

[1] UNIV WARWICK,DEPT BIOL SCI,GIBBET HILL RD,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

[2] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-11-2705

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The bacteriophage phiKP is capable of generalized transduction in Erwinia carotovora subspecies carotovora (Ecc) strains SCRI193 and ATCC 39048. PhiKP is a virulent phage containing double stranded DNA of approximately 46 kb. The frequencies of transduction were established for a number of chromosomal markers and plasmid pHCP2, and conditions for transduction optimized after exposure of the phage lysate to UV irradiation.

引用

收藏

页码：2705 / 2709

页数：5

相关论文

共 20 条

[1]

ARBER W, 1983, LAMBDA, V2, P433

[2] ULTRASTRUCTURE OF BACTERIOPHAGES AND BACTERIOCINS [J].

BRADLEY, DE .

BACTERIOLOGICAL REVIEWS, 1967, 31 (04) :230-+

[3]

CHATTERJEE AK, 1985, APPL ENVIRON MICROB, V50, P1

[4]

CHATTERJEE AK, 1981, J BACTERIOL, V143, P1444

[5] ANALYSIS OF LAMBDA-RECEPTOR AND BETA-LACTAMASE SYNTHESIS AND EXPORT USING CLONED GENES IN A MINI-CELL SYSTEM [J].

CLEMENT, J ;

PERRIN, D ;

HEDGPETH, J .

MOLECULAR AND GENERAL GENETICS, 1982, 185 (02) :302-310

[6] THE USE OF TRANSPOSON TN5 MUTAGENESIS IN THE RAPID GENERATION OF CORRELATED PHYSICAL AND GENETIC MAPS OF DNA SEGMENTS CLONED INTO MULTICOPY PLASMIDS - A REVIEW [J].

DEBRUIJN, FJ ;

LUPSKI, JR .

GENE, 1984, 27 (02) :131-149

[7] BACTERIOPHAGE LAMBDA-MEDIATED TRANSPOSON MUTAGENESIS OF PHYTOPATHOGENIC AND EPIPHYTIC ERWINIA SPECIES IS STRAIN DEPENDENT [J].

ELLARD, FM ;

CABELLO, A ;

SALMOND, GPC .

MOLECULAR & GENERAL GENETICS, 1989, 218 (03) :491-498

[8] CLONING OF THE CYSB GENE OF ERWINIA-CAROTOVORA SUBSP CAROTOVORA, AND THE IDENTIFICATION OF ITS PRODUCT [J].

HINTON, J ;

PEROMBELON, MCM ;

SALMOND, GPC .

MOLECULAR & GENERAL GENETICS, 1987, 207 (2-3) :466-470

[9] USE OF TNPHOA TO ENRICH FOR EXTRACELLULAR ENZYME MUTANTS OF ERWINIA-CAROTOVORA SUBSPECIES CAROTOVORA [J].

HINTON, JCD ;

SALMOND, GPC .

MOLECULAR MICROBIOLOGY, 1987, 1 (03) :381-386

[10] ISOLATION AND CHARACTERIZATION OF TRANSPOSON-INDUCED MUTANTS OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA EXHIBITING REDUCED VIRULENCE [J].

HINTON, JCD ;

SIDEBOTHAM, JM ;

HYMAN, LJ ;

PEROMBELON, MCM ;

SALMOND, GPC .

MOLECULAR AND GENERAL GENETICS, 1989, 217 (01) :141-148