EVALUATION OF THE GENOTOXICITY OF ENVIRONMENTAL CONTAMINANTS IN SEDIMENTS TO RAINBOW-TROUT HEPATOCYTES

被引:60
作者
GAGNE, F
BLAISE, C
机构
[1] Environment Canada, St. Lawrence Centre, Montréal, Quebec, H2Y 2E7
来源
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1995年 / 10卷 / 03期
关键词
D O I
10.1002/tox.2530100309
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Rainbow trout hepatocytes were used as an in vitro bioassay to assess the genotoxic potential of single chemicals and marine sediment extracts. Freshly prepared trout hepatocytes were exposed to either benzo[a]pyrene, N-methyl-N'-nitro-N-nitrosoguanidine, beta-naphtoflavone, or organic extracts of marine sediments for 24 h at 15 degrees C, Genotoxicity was assayed using the nick translation assay, wh ich makes use of a nonradioactive nucleotide (biotin-dUTP), and the DNA alkaline precipitation assay followed by fluorometric detection of DNA strands. Exposure to benzo[a]pyrene or methyl-N'-nitro-N-nitrosoguanidine, known indirect- and direct-acting genotoxins respectively, produced genotoxicity to rainbow trout hepatocytes with both assays. beta-Naphtoflavone displayed genotoxic activity in trout hepatocytes. Sediment extracts and reference sediment extracts displayed high toxicity and genotoxicity to trout hepatocytes. Chemical analyses showed that these sediments contained significant amounts of organochlorine pesticides, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons. Cell toxicity was correlated with total levels of organochlorine pesticides and polychlorinated biphenyls but not total levels of polycyclic aromatic hydrocarbons, No positive correlation was found with the nick translation assay between total levels of chemicals and genotoxicity in marine sediments. Genotoxicity obtained with the alkaline precipitation assay was correlated with levels of the organochlorine pesticide DDT. However, more tests would be required to further substantiate possible links with other specific chemicals. (C) 1995 by John Wiley and Sons, Inc.
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页码:217 / 229
页数:13
相关论文
共 30 条
[1]   MICRONUCLEUS TEST IN FISH FOR MONITORING THE GENOTOXIC EFFECTS OF INDUSTRIAL-WASTE PRODUCTS IN THE BALTIC SEA, SWEDEN [J].
ALSABTI, K ;
HARDIG, J .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1990, 97 (01) :179-182
[2]   CYTOTOXICITY AND GENOTOXICITY ASSAYS WITH CULTURED FISH CELLS - A REVIEW [J].
BABICH, H ;
BORENFREUND, E .
TOXICOLOGY IN VITRO, 1991, 5 (01) :91-100
[3]   ISOLATED FISH HEPATOCYTES - MODEL SYSTEMS FOR TOXICOLOGY RESEARCH [J].
BAKSI, SM ;
FRAZIER, JM .
AQUATIC TOXICOLOGY, 1990, 16 (04) :229-256
[4]   THE CYTOKINESIS-BLOCK MICRONUCLEUS TECHNIQUE - A DETAILED DESCRIPTION OF THE METHOD AND ITS APPLICATION TO GENOTOXICITY STUDIES IN HUMAN-POPULATIONS [J].
FENECH, M .
MUTATION RESEARCH, 1993, 285 (01) :35-44
[5]  
FOUQUET A, 1994, DIRECTIVES ASSURANCE
[6]   DETECTION OF DNA FRAGMENTATION IN APOPTOSIS - APPLICATION OF INSITU NICK TRANSLATION TO CELL-CULTURE SYSTEMS AND TISSUE-SECTIONS [J].
GOLD, R ;
SCHMIED, M ;
ROTHE, G ;
ZISCHLER, H ;
BREITSCHOPF, H ;
WEKERLE, H ;
LASSMANN, H .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (07) :1023-1030
[7]   INDUCTION OF PROPHAGE LAMBDA BY CHLORINATED PESTICIDES [J].
HOUK, VS ;
DEMARINI, DM .
MUTATION RESEARCH, 1987, 182 (04) :193-201
[8]   TROUT HEPATOCYTE CULTURE - ISOLATION AND PRIMARY CULTURE [J].
KLAUNIG, JE ;
RUCH, RJ ;
GOLDBLATT, PJ .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1985, 21 (04) :221-228
[9]   GENOTOXICITY IN WATER AND SEDIMENT EXTRACTS FROM THE ST-LAWRENCE-RIVER SYSTEM, USING THE SOS CHROMOTEST [J].
LANGEVIN, R ;
RASMUSSEN, JB ;
SLOTERDIJK, H ;
BLAISE, C .
WATER RESEARCH, 1992, 26 (04) :419-429
[10]   SISTER CHROMATID EXCHANGE TEST DETECTION OF TOXIN-INDUCED DAMAGE IN CULTURED FISH [J].
LOBILLO, J ;
DELGADO, JV ;
RODERO, A .
GENETICS SELECTION EVOLUTION, 1991, 23 :S160-S162